Chromatograms: isothermal vs programmed, isocratic vs gradient
The same mixtures run under two conditions. Late peaks in isothermal GC and isocratic HPLC are broad and low; temperature programming or a gradient sharpens them.
The same mixtures run under two conditions. Late peaks in isothermal GC and isocratic HPLC are broad and low; temperature programming or a gradient sharpens them.