Chemistry Labs
Undergraduate · 20 min

Identify an unknown liquid from its IR spectrum

Five unlabelled spectra of common solvents: find the diagnostic bands, deduce the functional group, and name the compound.

Goal

Assign bands near 3300 (O–H), ~3000 (C–H), 1650–1750 (C=O), 1050–1250 (C–O) and below 900 (fingerprint/out-of-plane bends) to identify each sample.

Apparatus and reagents

FTIR spectrometer with an ATR accessory, dropper bottles of ethanol, acetone, ethyl acetate, acetic acid and toluene.

Procedure

  1. Sample A shows a very broad band near 3340 cm⁻¹ and a strong one near 1050 cm⁻¹ but no carbonyl — deduce the functional group.
  2. Samples B and C both show C=O: compare the carbonyl position (≈1715 vs ≈1740 cm⁻¹) and look for strong C–O bands.
  3. Sample D pairs a huge, very broad O–H band spanning 3300–2400 cm⁻¹ with C=O near 1710 cm⁻¹ — a carboxylic acid dimer signature.
  4. Sample E has C–H stretches just above 3000 cm⁻¹, ring bands at 1605/1495 cm⁻¹ and strong out-of-plane bends at 728/694 cm⁻¹ — an aromatic compound.

What to observe

  • A broad O–H without C=O means an alcohol; O–H plus C=O means a carboxylic acid; C=O plus strong C–O bands means an ester.
  • Carbonyl frequency tracks the environment: ketone ≈1715, carboxylic acid ≈1710, ester ≈1740 cm⁻¹ in these samples.

Explanation

Vibrational frequency follows Hooke’s law: ν̃ ∝ √(k/μ) — strong bonds and light atoms give higher wavenumbers. Answers: A = ethanol (broad O–H, C–O 1050), B = acetone (C=O 1715, no O–H), C = ethyl acetate (C=O 1742, strong C–O 1240/1048), D = acetic acid (very broad O–H + C=O 1712), E = toluene (aromatic C–H >3000, ring modes, 728/694 bends).

History of the experiment

In 1859–60 Bunsen and Kirchhoff showed that every element writes its own barcode of spectral lines, reading caesium and rubidium out of mineral water within months. Ramsay used the same trick to complete the noble-gas family — argon, neon, krypton, xenon — between 1894 and 1898. Modern IR and NMR spectra, the kind matched in this lab, are the same idea at molecular resolution: a compound's vibrations and nuclear environments leave equally unique fingerprints.

Chemists behind it

Related topics

Virtual experiment: a simplified model to build intuition. It does not replace real lab work or safety training; never repeat chemistry at home without supervision.